how to operate colony counter(SA.microbiology) ep-01

Описание: SA.microbiology
A colony counter is a device that is frequently used to count bacterial or other microorganism colonies on a plate that contains a gelled growth medium.

Microbiologists have to tackle the daunting task of counting colonies, which can occasionally be undersized and obscured by the unique color of the culture media or the enumeration process itself. Thus, everyday routines require a better and more effective method of optimizing procedures.

A colony forming unit or CFU is a precise measurement to determine how many bacterial or mycelial cells are present in a given sample. Thus, the colony counting approach monitors viable CFUs, which can still reproduce via a process like binary fission under carefully monitored
Manual colony counters
Manual and semi-automatic counters rely on a technician’s ability to distinguish between colonies and mark them on the plate’s outside using a specific pen. The device maintains track of colonies with such markings. Although this approach somewhat aids, it is nonetheless time-consuming, chaotic, and prone to mistakes.

Parts and their functions of manual colony counters
Auto marker probe pen: A counter pen’s pressure on a colony causes a count to be registered by an audible beep and an advance on a computer screen.

Digital display: It reflects the total count made by the counter pen.

Lens: It helps in magnification.

Light source: A backlight source is used for illumination purposes.
Working Principle of Manual colony counters
A Petri plate is placed on an electronic pressure pad with light illumination, and each colony on the plate is marked by tapping the plate with an auto marker probe pen. A count is registered in the digital display by the touch’s pressure. This pressure can be altered based on the situation. It facilitates avoiding the error of overlooking or counting colonies twice. The equipment also has a graticule for Wolfheugal, a segmentation disc, and centering adapters for 50-90mm plates. Additionally, added features include darkening background for transparent colonies, glare-free illumination that allows for optimal peripheral colony viewing, and an integrated averaging tool for multiple plate counting.

Limitations of Manual colony counters
Labour intensive
Time-consuming
Relative low throughput
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SA.microbiology