Flow cytometry for extracellular vesicles: Edwin van der Pol & Joshua Welsh on MIFlowCyt-EV & more!
Автор: EVClub: Extracellular Vesicle Club
Загружено: 2021-07-19
Просмотров: 2736
Описание:
In this #EVClub, Josh Welsh and Edwin van der Pol present ISEV rigor and reproducibility initiatives around flow cytometric measurements of extracellular vesicles. These include, crucially, the MIFlowCyt-EV reporting framework, https://onlinelibrary.wiley.com/doi/f...
Dr. Welsh also presents a recent paper on calibration of scatter and fluorescence, https://onlinelibrary.wiley.com/doi/1...
Along the way, we also learned about YouTube instructions from Dave Gravano on converting text or image to fcs files: • Converting Text to a FCS File and Viewing ...
...found out more about EV/virus reference materials available from ViroFlow https://www.biorxiv.org/content/biorx... and others, such as the recombinant EVs from An Hendrix's group https://pubmed.ncbi.nlm.nih.gov/31337...
...and were pointed towards Dr. van der Pol's website for informative publications on membrane dyes and more. https://www.edwinvanderpol.com/public...
It was great to have input from Vera Tang and Joanne Lannigan, among others, during the extensive Q&A.
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