Gene Expression Profiling by High Quality Transcriptome Data | Protocol Preview

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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling - a 2 minute Preview of the Experimental Protocol

Vito M. Butardo Jr., Christiane Seiler, Nese Sreenivasulu, Markus Kuhlmann
Swinburne University of Technology, Department of Chemistry and Biotechnology; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Department of Molecular Genetics, Heterosis Research Group; International Rice Research Institute, Grain Quality and Nutrition Center, Applied Functional Genomics Cluster;

A method for transcriptome profiling of cereals is presented. The microarray-based gene expression profiling starts with the isolation of high-quality total RNA from cereal grains and continues with the generation of cDNA. After cRNA labelling and microarray hybridization, recommendations are given for signal detection and quality control.

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