Detecting Lyme Disease Spirochete, Borrelia Burgdorferi In Ticks Using Nested PCR l Protocol Preview

Описание: Watch the Full Video at https://www.jove.com/v/56471/detectin....

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR - a 2 minute Preview of the Experimental Protocol

Melanie K. B. Wills, Andrea M. Kirby, Vett K. Lloyd
Mount Allison University, Department of Biology;

Nested PCR is a sensitive, specific, and straightforward technique that can be applied to tick DNA extracts to probe for Borrelia burgdorferi, the causative agent of Lyme disease. The initial PCR experiment uses gene-specific primers to generate long amplicons, which then become templates for a subsequent reaction using internal primers.

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments