StrepPRO™ Streptococcal Grouping Kit

Описание: Hardy Diagnostics StrepPRO™ Grouping Kit provides a rapid latex agglutination method for the serological identification of Lancefield's groups A, B, C, D, F, and G from isolated colonies of beta-hemolytic Streptococcus spp. and Enterococcus faecalis.

StrepPRO™ Streptococcal Grouping Kit
https://hardydiagnostics.com/pl030hd

Features and Benefits:

• Accurate results
• Ready-to-use
• Easy-to-read results
• 98%+ sensitivity
• Only takes two minutes!

PROCEDURE

Specimen Collection:
For specific procedures regarding specimen collection and preparation of primary cultures consult standard microbiological references.

In general, an overnight, gram-positive, beta-hemolytic isolate of streptococcal isolate is required for use in this assay. Colonies should be chosen from an area demonstrating obvious isolation. One to four isolated colonies are recommended for grouping testing, however, if colonies are pinpoint, an increased number of colonies, approximately a loopful, should be used.

Test Protocol:
1. Allow all reagents to acclimatize to room temperature for 10 minutes prior to use.
2. Label one 12x75mm test tube for each specimen tested.
3. Add one drop of Extraction Reagent 1 to each tube.
4. Using a loop, select one to four isolated beta-hemolytic colonies and suspend them in Extraction Reagent 1. If colonies are miniscule, pick a loopful of colonies, such that the Extraction Reagent 1 becomes turbid.
5. Add one drop of Extraction Reagent 2 to each tube.
6. Mix the reaction by tapping the tube with a finger for 10 seconds.
7. Add five drops of Extraction Reagent 3 to each tube. Mix the reaction as in step 6.
8. Prior to use, re-suspend the Blue Latex Reagents, by inverting the tubes. Dispense one drop of each blue latex suspension onto separate circles on the test card.
9. Using a pasteur pipette, place one drop of the extract suspension next to the drop of latex suspension in each circle. Ensure that the pipette tip does not touch the latex suspension.
10. Mix the blue latex and the extract with the mixing sticks provided, using the complete area of the circle. A new stick should be used for each reagent.
11. Gently hand rock the entire card, allowing the mixture to flow slowly within the ring area.
12. At one minute, under normal lighting conditions, observe for agglutination, or strong clumping, of the blue latex particles. See Interpretation of Results for more information.

INTERPRETATION OF RESULTS

Positive Results:
A rapid and significantly strong clumping of the blue latex particles, to form an agglutination pattern, with only one of the latex reagents indicates an identification of the streptococcal isolate for the Lancefield group.

A weak reaction with a single blue latex reagent should be repeated using a heavier inoculum. The repeated test is considered positive if a visible agglutination occurs with only one of the blue latex reagents.

Negative Results:
No visible agglutination of the latex particles indicates a negative reaction for the particular Lancefield group.

StrepPRO™ Grouping Kit Instruction for Use (IFU):
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