MICROPROPAGATION TECHNOLOGY || BSC AGRICULTURE 6TH SEMESTER || COMPLETE GUESS PAPER
Автор: Bright Agro Classes
Загружено: 2025-06-18
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Описание:
Micropropagation Technology | Complete Guess Paper | BSc Agriculture 6th Sem || complete guess paper by bright agro classes Micropropagation Technology Full Lecture | BSc Agri 6th Semester || BSc Agri 6th Sem | Micropropagation Technology Full Revision || Micropropagation guess paper | Plant Tissue Culture | BSc Ag Classes
Micropropagation | B.Sc Agri Semester 6 | Bright Agro Classes
Micropropagation Technology in Plant Tissue Culture | guess paper
Plant Tissue Culture & Micropropagation | Complete Notes
BSc Agriculture Micropropagation | Complete Syllabus Covered
Micropropagation Technology | Fast T Revision for Pre-PG/AAO
Micropropagation Complete Lecture in Hindi | Agriculture 6th Sem @Bright_agro_classes
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Course Overview:
Micropropagation Technology (GPB-322) is a specialized course in the 6th semester of BSc Agriculture, focusing on plant tissue culture techniques, in vitro propagation methods, and their applications in plant breeding and biotechnology. This course provides theoretical and practical knowledge about the mass production of plants using aseptic techniques under controlled conditions.
Detailed Syllabus Analysis:
1. Introduction to Micropropagation:
Definition and scope of micropropagation
Importance of tissue culture in agriculture
Historical developments in plant tissue culture
2. Basics of Plant Tissue Culture:
Totipotency and its significance
Composition and preparation of culture media (MS, White’s, B5, etc.)
Sterilization techniques (autoclaving, surface sterilization)
Role of growth regulators (Auxins, Cytokinins, Gibberellins, etc.)
3. Micropropagation Techniques
Stages of micropropagation (Initiation, Multiplication, Rooting, Acclimatization)
Meristem culture and production of virus-free plants
Somatic embryogenesis and organogenesis
Callus culture and suspension culture
4. Applications of Micropropagation:
Large-scale clonal propagation
Germplasm conservation through tissue culture
Production of synthetic seeds
Role in crop improvement and genetic transformation
5. Commercial Aspects of Micropropagation:
Economic feasibility and commercial viability
Establishment of commercial tissue culture laboratories
Certification and quality control in tissue culture industries
6. Somaclonal Variation and Genetic Stability:Causes and detection of somaclonal variations
Applications in crop improvement
Molecular techniques for stability assessment
7. Hardening and Field Establishment:
Process of hardening and greenhouse acclimatization
Factors affecting survival rate of tissue-cultured plants
Field transfer and management of propagated plants
8. Biotechnological Advances in Micropropagation:
Role of molecular markers in tissue culture
Genetic transformation and gene editing in micropropagation
Practical Aspects:
Preparation of culture media (Murashige & Skoog, White’s medium)
Aseptic techniques – Surface sterilization, handling explants
Callus induction and differentiation
Micropropagation of selected crops (Banana, Sugarcane, Orchids, Potato, etc.)
Somatic embryogenesis and synthetic seed production
Acclimatization and hardening of micropropagated plants
Visit to commercial tissue culture laboratories
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