Chris Robinson - Dynamics Within The Honey Bee Microbiome Via Longitudinal Metagenomics | MVIF45 S05

Описание: Title of the presentation: Evolutionary Dynamics Within The Honey Bee Gut Microbiome Via Longitudinal Metagenomics

Speaker: Chris Robinson

More info on this event: https://www.microbiome-vif.org/en-US/...

Join us at the next meeting (free registration!): https://www.microbiome-vif.org/

And you can join us on Twitter:   / microbiomevif  

Abstract of this talk: Host-associated microbial communities are shaped by a combination of evolutionary and ecological processes. Within honey bees, broad-scale ecological dynamics of bee-associated bacteria have been extensively studied through 16S rRNA gene-based analyses. However, little is known about how honey bee-associated bacteria evolve over time. Here, we utilize longitudinally-sampled metagenomics from 12 individual honey bee colonies to quantify the ecological and evolutionary dynamics of greater than 300 bacterial species within and across honey bee colonies over the course of one year. We find that honey bee microbial communities are highly specific to the colony of origin, and that measures of ecological and evolutionary diversity are highly stable with respect to changes in season. Adapting standard population genetic models of evolutionary processes, we find that bee-associated microbial species accumulate genetic differences within bees that can be reliably distinguished from incidents of strain replacement. By tracking high-quality polymorphic alleles, we find that bacterial species commonly generate myriad, smaller evolutionary changes, including nucleotide variants that rapidly sweep to high frequency. We further show that the majority of these sweeps cannot be explained by stochastic processes (e.g. drift). Finally, we provide evidence that while purifying selection is dominant within the honey bee gut microbiome, a large number of these allelic sweeps occur simultaneously in spatially and taxonomically distant bacterial hosts, highlighting potential convergent, adaptive responses in the honey bee gut microbiome.