How To Perform A Quantitative ELISA

Описание: *CORRECTION: At 10:40, note that the secondary antibody binds to the primary antibody, not to the antigen*

This video demonstrates a quantitative #ELISA (enzyme-linked immunosorbent assay) using the Bio-Rad Explorer ELISA Immuno Explorer Kit. Serial dilutions of standards are performed and used for quantitation of test samples. Assay steps, including the addition of samples to the microtiter plate strip and washing steps, are shown. This technique may also be used for the Bio-Rad Explorer Giant Panda Problem Kit.

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