BiFC Assay For Protein-Protein Interaction: Onion Cells Using Helios Gene Gun l Protocol Preview

Описание: Watch the Full Video at https://www.jove.com/v/1963/bimolecul....

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun - a 2 minute Preview of the Experimental Protocol

Courtney A. Hollender, Zhongchi Liu
University of Maryland, Dept. Of Cell Biology and Molecular Genetics;

This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments