Encoding with Microfluidics and Applications

Описание: Presented By: Lydia L. Sohn, PhD

Speaker Biography: Lydia L. Sohn received her A.B. in Physics and Chemistry (1988), A.M. in Physics (1990), and Ph.D. in Physics (1992) from Harvard University. She was an NSF/NATO postdoctoral fellow at Delft University of Technology (1992-1993) and a postdoctoral fellow at AT&T Bell Laboratories (1993-1995) prior to joining the Physics faculty at Princeton University in 1995. Since 2003, Sohn has been a Professor in the Mechanical Engineering Department at UC Berkeley, where she now holds the Maynard Chair in Mechanical Engineering and is a Core Member of the UCSF-UC Berkeley Graduate Program in Bioengineering. Her work focuses on developing platforms to investigate susceptibility and resilience of disease. Sohn has received numerous awards, including the NSF CAREER and the Army Research Office Young Investigator Award. In 2010, Sohn received the prestigious W. M. Keck Foundation Medical Research award, and in 2014, Sohn’s developed label-free method—Node-Pore Sensing (NPS)—was named one of five “Revolutionary Platform Technologies for Advancing Life Sciences Research” by 6 major foundations. Sohn is a member of the Women in Cell Biology Committee of the American Society of Cell Biology and is a Fellow of the American Institute for Medical and Biological Engineering.

Webinar: Encoding with Microfluidics and Applications

Webinar Abstract: Flow cytometry is one of the cornerstones of biomedical research and clinical diagnostics. With its ability to screen individual cells for multiple protein epitopes simultaneously and subsequently identify sub-populations of cells, flow cytometry has had a profound impact in a broad range of areas including immunology, cancer, and regenerative medicine. Still flow cytometry is not without its challenges including coincidence events, irreversible antibody binding which can potentially alter cell properties, and high shear forces which can damage cells. We have developed an all-electronic method to screen cells for the immunophenotypic and mechanophenotypic profile label and camera free. Our method, Node-Pore Sensing (NPS), involves using a four-terminal measurement to measure the modulated current pulse caused by a cell transiting a microfluidic channel that has been segmented by a series of inserted nodes. As the modulated current pulse reflects the geometry of the NPS channel, we can in fact, encode spatial and temporal information to tease out coincidence events. As we use microfluidics, shear forces are low in our device. I will describe NPS and touch upon applications from measuring HIV viral particles in serum to immunophenotyping acute myeloid leukemic blasts to mechanophenotyping primary human mammary epithelial cells.

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