Interherence webinar: VAHEAT as a precise temperature reference during imaging

Описание: Everything in nature is in one way or another dependent on temperature. That's why scientific experiments need precise temperature control and calibration. In this webinar, Jaroslav Icha from Interherence briefly talked about our miniaturized stage top incubator VAHEAT, which is the only device that controls the temperature directly in the field-of-view. Our three academic speakers introduced examples of experiments from their research, which benefited from precise temperature calibration with VAHEAT.

Andrew York (Calico, USA) talked about a completely new type of fluorescent biosensors that his team has developed. These protein sensors are based on measuring fluorescence relaxation. The relaxation rate depends besides the measured quantity, like pH, also on temperature. It is important to calibrate their response to extract meaningful and reproducible data from these experiments. Then, the relaxation sensors present a very powerful technology especially for in vivo applications.

Guillaume Baffou (Institut Fresnel, France) presented experiments with thermobleaching and photobleaching of nanoparticles that were carried out during a project focused on microscale thermophoresis in liquids assisted by laser heating of gold nanoparticles. Additionally, he presented work focused on measuring temperature-dependent refractive index changes in liquids.

Pascal Lorentz (University of Basel, Switzerland) talked about using VAHEAT as a temperature calibration tool in the context of a light microscopy facility. To check the long term stability of incubation systems as well as to capture fast processes like illumination-induced heating.