MHC I Tetramer Staining used for Quantification of CD8+ T Cells | Protocol Preview

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Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector - a 2 minute Preview of the Experimental Protocol

Sarah Wilmschen, Zoltan Banki, Dorothee von Laer, Janine Kimpel
Medical University of Innsbruck, Division of Virology, Department of Hygiene, Microbiology, Social Medicine;

Here, we present a protocol for the ex vivo qualitative detection of antigen-specific CD8+ T cells. Analysis is possible with single cell suspensions from organs or from small amounts of blood. A broad range of studies require the analysis of cytotoxic T cell responses (vaccination and cancer immunotherapy studies).

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