Enhanced Reduced Representation Bisulfite Sequencing-Assessment: DNA Methylation l Protocol Preview

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution - a 2 minute Preview of the Experimental Protocol

Francine E. Garrett-Bakelman, Caroline K. Sheridan, Thadeous J. Kacmarczyk, Jennifer Ishii, Doron Betel, Alicia Alonso, Christopher E. Mason, Maria E. Figueroa, Ari M. Melnick
Weill Cornell Medical College, Department of Medicine; Weill Cornell Medical College, Institute for Computational Biomedicine; Weill Cornell Medical College, Department of Physiology and Biophysics; University of Michigan, Department of Pathology;

Enhanced Reduced Representation Bisulfite Sequencing is a method for the preparation of sequencing libraries for DNA methylation analysis based on restriction enzyme digestion combined with cytosine bisulfite conversion. This protocol requires 50 ng of starting material and yields base pair resolution data at GC-rich genomic regions.

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