Making Custom Database and Sequence Alignment using NCBI BLAST+

Описание: A simple video tutorial to construct a custom protein/nucleotide database, sequence retrieval, and pairwise sequence alignment using NCBI BLAST+.

Download latest BLAST+ — https://ftp.ncbi.nlm.nih.gov/blast/ex...

Creating BLAST Protein Database
===========================
./makeblastdb -in DB.fasta -out DB -dbtype prot -parse_seqids

Displaying List of Records in the Database
==================================
./blastdbcmd -db DB -entry all -outfmt "OID: %o GI: %g ACC: %a IDENTIFIER: %i"

Displaying Contents in the Database
=============================
./blastdbcmd -db DB -entry all

Searching Sequence from the Database
================================
./blastdbcmd -db DB -entry Sequence1

Retrieving Sequence from the Database
================================
./blastdbcmd -db DB -entry Sequence1 -out Sequence1.fasta

Performing Pairwise Alignment using BLAST+
=====================================
echo sequence | ./blastp -db DB

Saving the BLAST+ Pairwise Alignment Result
=====================================
echo sequence | ./blastp -db DB -outfmt 0 -out output.html -html

Identifiers:-
BLAST Ordinal Identifiers (BL_ORD_ID)
General (GNL)
Local (LCL)

---------------------------------------------------------------------------------------
My Blog: https://www.biob.in
Website: http://www.biogem.org
My Vlog:    / ashokkumarbioit  
YouTube Handle:    / @akbit  
My GitHub: https://github.com/AshokHub
---------------------------------------------------------------------------------------

#BioGem #biogem.org #BioBIN #biob.in #bioinformatics #AKBIT #ncbi #BLAST #NCBI_BLAST #bioinformaticsforbeginners #alignment #protein #dna #rna #nucleotides #nucleotide #comparision #sequence #tools #onlinetools #tutorial #tutorialvideo #protocol #database #LearnWithAKBIT