The RealTime-Glo MT cell viability assay - monitoring cell viability over time in the same well

Описание: When assessing dose-response, multiple plates of cells, media, reagents, and expensive treatments, can be reduced to a single plate using the RealTime-Glo MT cell viability assay.

Viability assay development using an endpoint method requires multiple plates.

Assay development using a real-time method uses only one plate, and much less reagent, to measure cell viability continuously out to 72 hours using a simple plate reading luminometer.

It's as easy as adding the reagent at any point during the assay and measuring luminescence as many times as desired for the same well.

The MT cell viability substrate enters the cell and only metabolically active cells can reduce substrate, which exits the cell and becomes a substrate for luciferase. Dead cells cannot reduce the substrate. Therefore, there is no luminescence.

With RealTime-Glo there is no guessing at optimal time points to capture response.

Data is collected in real-time so you never miss out. Since the cells are not lysed you can respond to what you see in real-time by using the same sample to measure cell death with an assay like CellTox Green, or measure apoptosis stress response markers or even analyse RNA.

The RealTime-Glo MT cell viability assay allows you to collect more relevant data per sample, with less time and effort, using fewer plates of cells.

To read more please visit https://www.promega.co.uk/products/ce...