Practical Guide for DNA Isolation from Plants | Step-by-Step CTAB Method for Beginners
Автор: Molecular Plant Science
Загружено: 2026-02-24
Просмотров: 6
Описание:
DNA isolation is the foundation of molecular biology and plant biotechnology research 🌱🧬
In this practical guide, you’ll learn how to extract high-quality DNA from plant tissues using the CTAB method — one of the most reliable protocols for plants rich in polysaccharides and secondary metabolites.
This step-by-step tutorial covers:
🌿 Sample collection and preparation
🧪 Preparation of CTAB extraction buffer
🔬 Cell lysis and incubation
🧬 Chloroform:isoamyl alcohol purification
🧫 DNA precipitation using cold isopropanol
💧 Washing with ethanol
📊 DNA quantification and quality check
Whether you're a biology student, researcher, or lab beginner, this video will help you perform plant DNA extraction confidently and accurately.
📌 Applications include:
PCR amplification
Genome sequencing
Molecular marker analysis
Genetic diversity studies
Plant breeding research
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📢 Hashtags
#DNAIsolation
#PlantDNA
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