Western Blot: Introduction to Electroblotting

Описание: The western blot is a widely used research technique that detects proteins using antibodies. This video will cover the theory and the details behind the process of electroblotting. Electroblotting refers to the process of transferring proteins from the gel onto the membrane.

This video was made by McMaster University students Aniqa Chaudhry, Brooke D'Mello, Ibrahim Ilahi, Kunwar Karim, Mubariz Maqsood, Adnan Moinuddin, and Julia Zhu in collaboration with the Demystifying Research McMaster Program.

Episode #1: Introduction to Western Blotting:    • Introduction to the Western Blot  

Episode #2: SDS-PAGE:    • Western Blot: Introduction to SDS-PAGE  

Episode #3: Wet Transfer:    • Western Blot: How to do a Wet Transfer  

Episode #5: Blocking:    • Western Blot: Introduction to Blocking  

Episode #6: Immunodetection:    • Western Blot: Introduction to Immunodetection  

Episode #7: Results & Analysis:    • Western Blot: Result Interpretation  

This video is for general and educational purposes only.

Subscribe to the Demystifying Research McMaster channel.

Copyright McMaster University 2022

#westernblot #electroblotting

References:

A good sandwich must be tight - change your blotting pads. (2016, May 17). Advansta Inc. https://advansta.com/good-sandwich-mu...

Arslan, J. (2019, December 2). Wet or dry protein transfer: Which one to choose for your western blotting? Novusbio.Com. https://www.novusbio.com/antibody-new...

Bass, J. J., Wilkinson, D. J., Rankin, D., Phillips, B. E., Szewczyk, N. J., Smith, K., & Atherton, P. J. (2017). An overview of technical considerations for Western blotting applications to physiological research. Scandinavian Journal of Medicine & Science in Sports, 27(1), 4–25. https://doi.org/10.1111/sms.12702

Bio-Rad. (n.d.). Western blot protocol. Bio-Rad. Retrieved April 23, 2022, from https://www.bio-rad-antibodies.com/we...

Gibbons J. (2014). Western blot: protein transfer overview. North American journal of medical sciences, 6(3), 158–159. https://doi.org/10.4103/1947-2714.128481

Goldman, A., Harper, S., & Speicher, D. W. (2016). Detection of proteins on blot membranes. Et al [Current Protocols in Protein Science], 86(1), 10.8.1-10.8.11. https://doi.org/10.1002/cpps.15

Kurien, B. T., & Scofield, R. H. (2015). Western blotting: an introduction. Methods in Molecular Biology (Clifton, N.J.), 1312, 17–30. https://doi.org/10.1007/978-1-4939-26...

Mahmood, T., & Yang, P.-C. (2012). Western blot: technique, theory, and trouble shooting. North American Journal of Medical Sciences, 4(9), 429–434. https://doi.org/10.4103/1947-2714.100998

Pettegrew, C. J., Jayini, R., & Islam, M. R. (2009). Transfer buffer containing methanol can be reused multiple times in protein electrotransfer. Journal of Biomolecular Techniques: JBT, 20(2), 93–95.

Protein Transfer Methods for Western Blot. (n.d.). Sinobiological.Com. Retrieved April 23, 2022, from https://www.sinobiological.com/catego...

Systems, L. B. T. (2018, January 31). Wet Tank or Semi Dry Transfer: What’s best for Western blotting? https://blog.btlabsystems.com/blog/we...

The Protein Man. (2014, November 12). PVDF or Nitrocellulose - Which Membrane is Best? Gbiosciences.Com. https://info.gbiosciences.com/blog/bi...

Towbin, H., Staehelin, T., & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4350–4354. https://doi.org/10.1073/pnas.76.9.4350

Western blot transfer methods - US. (n.d.). Retrieved April 23, 2022, from https://www.thermofisher.com/ca/en/ho...