Budding Yeast Protein Extraction & Purification: Studying Function, Interactions l Protocol Preview

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Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications - a 2 minute Preview of the Experimental Protocol

Eva Paige Szymanski, Oliver Kerscher
The College of William & Mary, Integrated Science Center, Biology Department;

The preparation of high quality yeast cell extracts is a necessary first step in the analysis of individual proteins or entire proteomes. Here we describe a fast, efficient, and reliable homogenization protocol for budding yeast cells that has been optimized to preserve protein functions, interactions, and post-translational modifications.

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