An improved solution for generating large CRISPR HDR knock-ins
Автор: Integrated DNA Technologies (IDT)
Загружено: 2022-03-22
Просмотров: 2160
Описание:
Introducing Alt-R™ HDR Donor Blocks and Alt-R HDR Enhancer V2
Learn more: https://idtb.io/jz8so8
CRISPR-based homology-directed repair (HDR) is an invaluable tool to facilitate specific mutations in a genomic region of interest in research studies. While many methods have been reported for improving HDR efficiency, achieving precise changes via HDR remains a challenge, particularly for large knock-ins. These insertions can be generated via HDR using enzymatically generated donor templates.
What you will learn:
1. How dsDNA donor templates with novel end-modifications improve the frequency of HDR and reduce homology-independent (blunt) insertion events.
2. How the addition of Alt-R HDR Enhancer V2, a small molecule compound, can further improve HDR rates.
3. Design considerations when using large dsDNA templates for successful HDR experiments.
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