Titration ELISA to Determine KD of Receptor Ligand Interaction | Protocol Preview
Автор: JoVE (Journal of Visualized Experiments)
Загружено: 2022-12-13
Просмотров: 953
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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction - a 2 minute Preview of the Experimental Protocol
Johannes A. Eble
University of Münster, Institute of Physiological Chemistry and Pathobiochemistry;
A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.
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