Spore Stain Procedure (Schaeffer-Fulton Method)
Автор: Sci- Inspi
Загружено: 2019-02-04
Просмотров: 86114
Описание:
Bacterial Endospore stain procedure. This protocol is taken from "Benson's Microbiological Applications: Laboratory Manual in General Microbiology" Eleventh Edition
1. Cover the bacteria smear with a piece of paper towel and saturate it with malachite green. Steam it over boiling water for 5 minutes and add more stain as it boils off.
2. Allow the slide to cool, remove the piece of paper towel and rinse for 30 seconds.
3. Counterstain with safranin for about 20 seconds.
4. Rinse briefly with water to remove safranin
5. Blot dry and view under the microscope in immersion oil.
Camera: Nikon D3300
Microscopes:
Leica ATC 2000 - 1000X
American Optical One - Ten microscope - 1500X
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