ELISA Kit Product Demonstration | Innovative Research

Описание: Join Maria, the VP of Sales for the Midwest Region at Innovative Research, as she guides you through a detailed demonstration of our Human IgG ELISA Kit. This video tutorial will walk you through each step of the ELISA process, ensuring you can achieve precise and accurate measurements of human IgG antigen levels in your samples. Whether you’re a seasoned expert or new to ELISA assays, you’ll find valuable insights and tips to enhance your lab skills.

In this video, you'll learn:

How to prepare and use the Human IgG ELISA Kit.
Step-by-step procedures from sample collection to data analysis.
Best practices for handling reagents and avoiding common mistakes.

#biotechnology #tutorial #researchmethods
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0:00-0:25 | Welcome!
0:25-0:55 | Unboxing & Kit Contents
0:55-1:07 | Before You Get Started...
1:07-1:20 | Preparing the Reagents
1:20-2:17 | Collecting the Sample
2:17-3:51 | Preparing the Standard
3:51-4:39 | Streptavidin-HRP Addition
4:39-5:17 | Add TMB Substrate
5:17-6:17 | Measure Absorbance & Analyze Data
6:17-6:48 | Thank You for Watching!

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The Human IgG ELISA Kit is tailored for the quantitative determination of total human IgG antigen in serum, plasma, and other biological fluids, using a simple strip well format with reagents sufficient for up to 96 tests. The assay is designed to measure levels of human IgG without distinguishing between subclasses, making it ideal for a wide range of research applications. After binding to a capture antibody on the microtiter plate, a horseradish peroxidase-labeled polyclonal anti-human IgG antibody is added. Following washing steps, TMB substrate is added for color development, which is read at 450 nm. The color intensity directly correlates with the IgG concentration in the sample.