analytica conference 2022 - Session: Perspectives in Small-Molecule NMR

Описание: analytica conference 2022 - Session: Perspectives in Small-Molecule NMR

Presentation:
Microfluidic Magnetic Resonance As A Tool To Study Biological Systems
Prof. Marcel Utz, University of Southampton
Microfluidic lab-on-a-chip (LoC) systems are rapidly gaining ground in the life sciences. In particular, they provide a convenient platform to integrate culture of cells, cell aggregates, tissues, and even small animals with downstream analytics such as immunostaining, proteomics, and PCR-based readouts. Nuclear magnetic resonance spectroscopy is an ideal tool to quantify metabolic processes in living systems since it is non-invasive, generic, and requires only minimal sample preparation.It is therefore possible to use NMR to obtain detailed and quantitative information on metabolic processes of live systemsin operando. This opens exciting possibilities, for example experimental designs that use analytical data to control experimental conditions in real-time feedback. Until recently, however, limited sensitivity and problems of systems integration have hindered the application of NMR in the contextof microfluidic culture devices. Recent progress in the design of NMR detector systems and in their integration with LoC devices has begun to change this. In this talk, the fundamental challenges that need to be overcome to fully integrate LoC technology and high-performance NMR spectroscopy will be discussed. Some recent examples will be presented, including cultures of adherent cells, cell spheroids, and liver tissue slices.
21.06.2022 13:30 - 14.00 ICM/Hall 2