Recombinant Cellulase Cel5A Expression | Protocol Preview
Автор: JoVE (Journal of Visualized Experiments)
Загружено: 2022-09-19
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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants - a 2 minute Preview of the Experimental Protocol
Megan Garvey, Johannes Klinger, Holger Klose, Rainer Fischer, Ulrich Commandeur
RWTH Aachen University, Institute for Molecular Biotechnology, Bio7; Fraunhofer Institute for Molecular Biology and Applied Ecology,;
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.
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