Quantile Normalization for Gene Expression (RNA-seq) on T BioInfo

Описание: Quantile normalization, and other normalization procedures are important to transform distributions between, and amongst, different samples to have the same distribution. Quantile normalization is a technique of mapping original values to the corresponding quantile of a standard distribution. Here, an estimation of the sequencing depth of an experiment is determined by an upper quartile of its counts.

Comparing quantile values instead of raw values will eliminate differences solely rooted in technical variation, such as the amount of RNA sequenced, sequencing machine type, or even unexplained differences that arise between different technicians following the same protocol. In addition, while normalizing data we will use a log transformation that makes it easier to interpret differences in gene expression.

Link to course material for Quantile Normalization for Gene Expression (RNA- Seq) on T-Bioinfo - https://learn.omicslogic.com/Learn/co...