Orbital Shaking Culture: Mammalian Cells In O-shaped Vessels l Protocol Preview

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An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates - a 2 minute Preview of the Experimental Protocol

Ikki Horiguchi, Ikumi Suzuki, Takashi Morimura, Yasuyuki Sakai
University of Tokyo, Department of Chemical System Engineering; Osaka University, Department of Biotechnology; Fukoku Co. Ltd, Biotech Business Unit; University of Tokyo, Institute of Industrial Science;

Here we present a protocol for using O-shaped vessels, specialized for suspension cultures of cellular aggregates, with orbital shaking. The HEK293 cells grown in this bag form more homogeneous aggregates than those grown in conventional culture vessels.

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