Acquiring Fluorescence Time-Lapse Movies Of Budding Yeast: GRAFTS l Protocol Preview

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Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS - a 2 minute Preview of the Experimental Protocol

Christopher J. Zopf, Narendra Maheshri
Massachusetts Institute of Technology, Department of Chemical Engineering;

We present a simple protocol to obtain fluorescence microscopy movies of growing yeast cells, and a GUI-based software package to extract single-cell time series data. The analysis includes automated lineage and division time assignment integrated with visual inspection and manual curation of tracked data.

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