Two-Photon Imaging Predicts Stress-Induced Anhedonia | Bruker Neuroscience Summit 2025

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𝗣𝗥𝗘𝗦𝗘𝗡𝗧𝗘𝗥
Austin Coley
Assistant Professor, Dept. of Neurobiology, Brain Research Institute, Integrative Center for Learning and Memory, University of California - Los Angeles

𝗔𝗕𝗦𝗧𝗥𝗔𝗖𝗧
The mental health field lacks granularity in diagnostic practices, which may contribute to the variability in efficacy of existing treatments. Anhedonia, a condition identified in multiple neuropsychiatric disorders, is described as the inability to experience pleasure and is linked to anomalous medial prefrontal cortex (mPFC) activity. The mPFC is responsible for higher order functions, such as valence processing; however, it remains unknown how mPFC valence-specific neuronal population activity is affected during anhedonic conditions. To test this, we implemented the unpredictable chronic mild stress (CMS) protocol in mice and examined hedonic behaviors following stress and ketamine treatment. We performed in vivo 2-photon calcium imaging to longitudinally track mPFC valence-specific neuronal population activity and dynamics during Pavlovian conditioning tasks.

Also, we utilized behavioral pose-estimation tracking systems to predict anhedonia based on facial features during Pavlovian conditioning tasks. Lastly, we applied a linear classifier to test whether we can decode susceptibility to chronic mild stress based on mPFC valence-encoding properties prior to stress-exposure and behavioral expression of susceptibility. These results indicate that mPFC valence encoding properties are predictive of anhedonic states. Altogether, these experiments point to the need for increased granularity in the measurement of both behavior and neural activity, as these factors can predict the induction conditions of stress-induced anhedonia.

#neuroscience #microscopy #optogenetics #anhedonia

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