laser axotomy system aimed at investigating axon regeneration in C. elegans

Описание: Reference: https://app.jove.com/t/3331/construct...
Developing an affordable laser axotomy system presents a unique opportunity to investigate axon regeneration in the model organism Caenorhabditis elegans. This innovative approach aims to create a cost-effective setup that allows researchers to precisely sever axons using laser technology, thereby facilitating the study of the subsequent regenerative processes. By utilizing readily available materials and components, the system can be constructed without the need for expensive laboratory equipment, making it accessible to a broader range of research institutions. The design focuses on achieving high precision in targeting specific axons while minimizing damage to surrounding tissues, which is crucial for obtaining reliable data on regeneration.

The methodology involves integrating a laser source with a microscope, enabling real-time visualization of the axons during the axotomy procedure. The system is calibrated to ensure that the laser's intensity and duration are optimized for effective axon cutting without causing excessive thermal damage. Additionally, the use of C. elegans as a model organism is particularly advantageous due to its well-mapped nervous system and the ease of genetic manipulation. This allows researchers to explore various genetic factors that may influence axon regeneration, providing insights into the underlying biological mechanisms.

Once the laser axotomy system is operational, researchers can conduct a series of experiments to assess the regenerative capabilities of axons in C. elegans. By employing various imaging techniques, such as fluorescence microscopy, they can monitor the growth of axons over time and analyze the effects of different genetic backgrounds or environmental conditions on regeneration. This low-budget system not only democratizes access to advanced research techniques but also contributes to a deeper understanding of neuronal repair processes, which could have implications for regenerative medicine in more complex organisms.