Neuromuscular Junction As The Model For Chemical Transmission (Neurophysiology) | Full Discussion

Описание: Welcome to Science With Tal!

In this video, we will go over the neuromuscular junction and the main experiments that have led us to use this structure as a model for chemical transmission.

To watch the first part of this discussion (Signal propagation in the Neuron) make sure to go to:    • Signal Propagation In The Neuron (Neurophy...  

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Timestamps

0:00 Introduction
0:10 Summary of previous discussion
1:10 What happens at the terminal
1:38 Electrical synapse overview
4:36 Chemical synapse overview
7:49 Electrical vs chemical synapses
8:15 Where is the NMJ from?
9:12 Why the NMJ?
10:17 NMJ terminology
11:06 Acetylcholine packaging process
12:15 Acetylcholine release
12:40 Nicotinic acetylcholine receptor overview
15:03 Acetylcholine degradation & muscle fiber response
17:02 End plate potential recordings (Katz experiments)
19:30 Mini end plate potential & statistical theory
25:45 Experimental basis for calcium influence (necessary & sufficient)
31:12 Experimental basis for exocytosis & endocytosis (Heuser & Reese)
37:50 Vesicle cycle as understood now
45:50 Patch clamp of nicotinic acetylcholine receptors & current analysis of NMJ
53:25 Equivalent circuit model of the NMJ
58:50 Opening on next section (CNS neurons & synapses)
1:01:10 Conclusion

Resources used

Here is a list of the resources that I’ve used to produce this video. (Author: title resource)

Dale Purves: Neuroscience (6th edition)
Eric Kandel: Principles of neural science (6th edition)
Robertson, J. David: Some Features of the Ultrastructure of Reptilian
Skeletal Muscle
Heuser, J E et al. : Synaptic vesicle exocytosis captured by quick freezing
and correlated with quantal transmitter release
Heuser, J E and T S Reese: Evidence for recycling of synaptic vesicle
membrane during transmitter release at the frog neuromuscular junction
Llinás, R and Y Yarom: Properties and distribution of ionic
conductances generating electroresponsiveness of mammalian inferior olivary neurones in vitro
Katz, B, and R Miledi: A study of synaptic transmission in the
absence of nerve impulses
BOYD, I A,and A R MARTIN: The end-plate potential in mammalian
muscle
DEL CASTILLO, J, and B KATZ: Quantal components of the end-plate potential

To have more information on these resources, you can refer to the conclusion section where a more formal citation is provided.

Video credits

Writing: Tal Klimenko
Voice: Tal Klimenko
Animations: Tal Klimenko
Drawings: Tal Klimenko
Editing: Tal Klimenko
Introductory jingle: Thierry Du Sablond
Conclusion music: lukrembo - jay (   • lukrembo - jay  )