Thin Layer Chromatography (TLC) Plate: How to Make Thin layer Chromatographic Plate

Описание: Thin Layer Chromatography (TLC): How to Make Thin layer Chromatographic Plate
Chromatography- Chromatography is a scientific technique to separate the mixture into its individual components.
Chromatography is derived from Greek Word: Khromatos meaning colour & grapho means writing/drawing or visual identification.
There are several types of chromatography like-
(1) Paper chromatography
(2) Thin layer chromatography
(3) Ion exchange chromatography
(4) HPLC- High-performance liquid chromatography
(5) GC- Gas chromatography
Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass or plastic, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase.

Readymade TLC plates: These are stable and chemically inert plates, where a thin layer of stationary phase is applied on its whole surface layer. The stationary phase on the plates is of uniform thickness and is in a fine particle size.

TLC Plate preparation consists of following steps-
1- Selection and cleaning of the glass plate for Thin-layer Chromatography (TLC)
2- Drying of plate Thin-layer Chromatography (TLC)
3- Making a slurry of silica gel with suitable solvent for Thin-layer Chromatography (TLC)
4- Transferring the slurry into a beaker
5- Transferring of slurry on a glass plate Thin-layer Chromatography (TLC)
6- Uniformly distribution of a slurry
7- Allow the slurry to dry at room temperature
8- Dry and activate a glass plate Thin-layer Chromatography (TLC)
The Thin-layer Chromatography (TLC) plates are then ready to use.