Ensemble Learning Algorithms to Predict Scaffold Matrix Attachment Regions

Описание: 250701
Department of Chemistry, Biochemistry & Physics
Bridging the broad spectrum of chemistry: Amino triester lipids as biodegradable surfactants for drug delivery and precise control of quantum dot formation

The delivery of anionic cargoes, including mRNA, small molecules, aptamers, and other oligonucleotide-based therapeutics, most fundamentally requires the enablement of a mono- or polyanionic payload to be delivered across a lipophilic membrane bilayer. Delivery systems typically rely on bifunctional cationic materials composed of a protonatable amine headgroup that electrostatically complexes with the desired anionic cargo, along with a lipid segment that permeates the hydrophobic lipid bilayer membrane. These materials may complex with anionic targets to form nanoparticles, liposomes, and other macromolecular structures, and these formulations have been previously described to be highly efficacious in mRNA vaccination gene delivery, siRNA delivery, and small molecular drug delivery. Given this, we synthesized–under nonhazardous, mild conditions– four non-toxic triester “tripod” lipids. Preliminary investigations demonstrated that these “tripod” lipids do not induce cell lysis, indicating potential for use in larger drug delivery systems. We then complexed the “tripod” lipids with calcium phosphate (CaP) in an attempt to create lipid-coated nanoparticles capable of localized drug delivery. Through a series of cell proliferation and fluorescence imaging assays conducted on lung and colorectal cancer cell lines, we evaluated the ability of our “tripod” lipids and our lipid-CaP conjugates in delivering clinically prevalent cancer therapeutic cargoes across the cell membrane. Lastly, we extended our study into the domain of inorganic chemistry by investigating the usage of our “tripod” lipids as surface ligands in the formation of quantum dots capable of fluorescent tagging in biological assays.
RESEARCHERS: Chancie Chou, Lynbrook High School '26
ADVISOR: Njoo Lab Synthesis | Physical Organic Chemistry | Catalysis | Chemical Biology | Spectroscopy | Medicinal Chemistry
KEYWORDS: Organic Synthesis | Drug Delivery