I tried NILE RED DNA extraction method BUT with a twist
Автор: Mr Chemical
Загружено: 2025-02-23
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🔬 *Extracting DNA from a Banana | Fun & Easy Science Experiment!* 🍌🧬
Have you ever wondered what DNA looks like? In today’s video, we’re extracting *real DNA* from a banana using simple household ingredients! This experiment is super easy and fun to try at home. 🏠🔍
📌 *What You’ll Learn in This Video:*
✔️ What DNA is and why it’s important
✔️ Why bananas are great for DNA extraction
✔️ Step-by-step process to extract DNA using salt, detergent, and ethanol
✔️ How DNA looks and feels when extracted!
✨ Materials Used:
✅ 1 Banana 🍌
✅ Plastic Bag (Not Ziplock)
✅ Water 💧
✅ Common Salt (NaCl) 🧂
✅ Dish Detergent 🧼
✅ Tea Strainer ☕
✅ Beaker 🧪
✅ Ethanol (C₂H₅OH) 🍶
🧪 Chemical Reactions Involved:
1️⃣ Breaking Cell Membranes with Detergent
The dish detergent contains surfactants that break down *phospholipid bilayers* of cell membranes and nuclear membranes, releasing DNA.
Reaction:
🫧 Lipid Bilayer + Detergent → Dissolved Membranes + Free DNA
2️⃣ Salt Helps DNA Clump Together:
Salt neutralizes the negative charge on DNA, allowing it to come together instead of staying dissolved in water.
Reaction:
🧂 Na⁺ ions from salt neutralize DNA’s negative phosphate backbone → DNA strands stick together*
3️⃣ Ethanol Precipitates DNA:
DNA is soluble in water but **insoluble in ethanol**. When ethanol is added, DNA becomes visible as a white, stringy substance.
Reaction:
🧪 *DNA (soluble in water) + Ethanol → DNA precipitate (visible!)
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Step-by-Step Guide:
🔹 Peeling and preparing the banana
🔹 Making an extraction solution (water + salt + detergent)
🔹 Mixing the solution with the banana mash
🔹 Filtering the extract
🔹 Adding ethanol to make DNA visible
🔹 Extracting and observing the slimy, thread-like DNA!
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💬 Try this experiment at home and let me know your results in the comments!** What should I extract DNA from next? 🤔
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I tried NILE RED DNA extraction method BUT with a twist #experiment #DNA
@MrChemical09
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