Droplet Digital PCR Using the Bio-Rad QX200™ and AutoDG™ ddPCR™ Systems

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Based on water-emulsion droplet technology, Droplet Digital™ PCR (ddPCR™) partitions a DNA sample into 20,000 nanoliter-sized droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplet gives precise, absolute target quantification.

From detection of rare mutations and cancer biomarkers to quantification of gene editing events and miniscule viral loads, the QX200™ Droplet Digital PCR System has been used to redefine the limits of the absolute nucleic acid quantification. ddPCR platforms have outperformed other digital PCR systems by several order of magnitude. The third-generation QX200™ AutoDG™ System now brings automation and scalability to digital PCR.

The ddPCR System lets you:
Detect rare DNA target copies with unmatched sensitivity
Determine copy number variation with unrivaled accuracy
Measure gene expression levels with the highest precision

Application and Uses
Copy number variation
Rare sequence detection
Mutation detection
Gene expression analysis
miRNA analysis
Next generation sequencing sample quantification
Genome edit detection"
From detection of rare mutations and cancer biomarkers to quantification of gene editing events and miniscule viral loads, the QX200™ Droplet Digital PCR System has been used to redefine the limits of the absolute nucleic acid quantification. ddPCR platforms have outperformed other digital PCR systems by several order of magnitude. The third-generation QX200™ AutoDG™ System now brings automation and scalability to digital PCR.

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