How to DESIGN PCR PRIMERS | From Sequence to Synthesis
Автор: Adwoa
Загружено: 2025-08-18
Просмотров: 386
Описание:
Learn how to design effective PCR and qPCR primers from start to finish! In this comprehensive tutorial, I’ll show you:
✅ Where to download your gene sequence (FASTA format)
✅ How to use online tools like Primer3, Primer-BLAST, and IDT PrimerQuest
✅ How to check that your primers bind to the target sequence
✅ How to detect and avoid secondary structures (hairpins, dimers)
✅ How to ensure primers don’t bind elsewhere in the genome
✅ How to order primers from a synthesis company
Whether you’re a beginner in molecular biology or an experienced scientist, this step-by-step guide will help you create specific, efficient, and reliable primers for your experiments.
📌 Tools & Resources Mentioned:
NCBI Sequence Database: https://www.ncbi.nlm.nih.gov
Primer3: http://primer3.ut.ee
IDT PrimerQuest: https://www.idtdna.com/PrimerQuest
NCBI Primer-BLAST: https://www.ncbi.nlm.nih.gov/tools/pr...
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