Western Blot Troubleshooting: How to Fix Common Problems & Failures

Описание: Optimized to hit all the "pain point" keywords researchers search for when an experiment goes wrong.

Is your Western Blot looking messy, or worse—completely blank? Don't panic! Western Blotting is a complex, multi-step process, and things can go wrong at any stage. In this comprehensive troubleshooting guide, we diagnose the most common Western Blot problems and provide proven solutions to get your results back on track.


We cover the "Big 4" Western Blot Issues:

No Signal / Weak Bands: Is it your primary antibody, protein degradation, or a transfer failure?

High Background: Dealing with "noisy" blots, splotchy backgrounds, and non-specific binding.

Ghost Bands & Nonspecific Bands: Why you’re seeing proteins at the wrong molecular weight.

Physical Artifacts: How to avoid bubbles, "smile" gels, and uneven transfer patterns.

What you will learn in this video:

How to verify your transfer with Ponceau S.

Why your blocking buffer (Milk vs. BSA) might be ruining your signal.

The importance of wash steps in reducing background noise.

How to check if your secondary antibody is still active.


#WesternBlot #Troubleshooting #MolecularBiology #LabLife #ScienceTutorial #ProteinAnalysis #Biotech