Split Gene: Exons & Introns | Splicing Mechanism | DSC 14 Cell & Molecular Biology | DU Zoology Sem5

Описание: This video explores the fundamental concept of Split Genes and RNA Splicing, an essential post-transcriptional process in eukaryotic gene expression. This is a key topic in DSC-14: Cell and Molecular Biology, part of the B.Sc. Zoology Semester 5 syllabus under the New Education Policy (NEP), University of Delhi.

Split genes contain non-coding introns interrupting the coding exons, and splicing ensures their precise removal. This video covers both self-splicing mechanisms and spliceosome-mediated splicing, integrating visual diagrams and clear scientific explanations to help you master this complex yet fascinating topic.

🔬 In this video, you’ll learn:

Split Genes: Definition, discovery (via studies on adenovirus), and significance in evolution
Introns vs Exons: Structure, function, and biological importance
Types of Introns: Group I, Group II, and spliceosomal — categorized based on their splicing mechanism
General Splicing Mechanism: Role of conserved sequences (5′ GU, 3′ AG, branch point) and two-step transesterification
Self-Splicing Introns:
Group I: Involvement of an external guanosine, no energy required
Group II: Internal A residue initiating lariat formation
Spliceosome-Mediated Splicing:
Role of snRNAs (U1, U2, U4, U5, U6) and snRNPs
Assembly and rearrangement of the spliceosome complex
Diagrams Explained: Stepwise annotated illustrations of Group I, Group II, and spliceosome pathways

This topic is vital for understanding how genetic information is edited before translation, allowing for tissue-specific protein expression and evolutionary innovation in eukaryotes.

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