Engineering Mushrooms with E. coli

Описание: So you want to learn how to engineer fungi and mushrooms diy-style from a home lab? Great! Learn E. coli transformation! Learning how to transform E. coli is not only practically useful for copying and assembling DNA plasmids in a process known as molecular cloning, but it's also a great introduction to the transformation process of more complex organisms like fungi.

This video covers the uses and importance of E. coli, including performing heat--shock transformation in the context of learning how to transform fungi to create new and exciting

CHAPTERS
0:00 Introductions
0:12 Food Tech
1:13 Mushroom Innovators
2:18 Future of Mushroom Engineering
2:51 Uses of E. coli
4:15 Protein Expression
5:30 Transformation Framework
5:55 E. coli Strains
7:17 E. coli Transformation Process
8:20 Preparing Reagents
8:41 Kanamycin Stock Prep
9:32 Seed Culture
10:47 Prepping Selection Plates
12:20 Ice Bath
12:37 Bacterial Pelletization
13:13 Adding CaCl2
13:41 Adding the Plasmid DNA
14:02 Heat Shock
14:27 Recovery
14:43 Plating
15:26 Transformation Results

Mossy Creek Mushrooms
  / mossycreekmushrooms  

Spencer's Shroomery
  / spencers_shroomery  

MycoSymbiotics
  / mycosymbiotics  

CONNECT:
Subscribe to this YouTube channel
LinkedIn:   / joshuamcginnis  
Instagram:   / everymanbio  
Website: https://everymanbio.com/
Patreon:   / everymanbio