TurboID-Based Proximity Labeling to identify Protein Interactions | Protocol Preview

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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks - a 2 minute Preview of the Experimental Protocol

Yongliang Zhang, Yuanyuan Li, Xinxin Yang, Zhiyan Wen, Ugrappa Nagalakshmi, Savithramma P. Dinesh-Kumar
China Agricultural University, State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences; University of California, Davis, Department of Plant Biology, College of Biological Sciences; University of California, Davis, Genome Center, College of Biological Sciences;

Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.

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