Decarboxylase Test | Principle, Procedure & Result Interpretation | Systematic Bacteriology | BMLS

Описание: 🧪 Decarboxylase Test | Principle, Procedure & Result Interpretation | Systematic Bacteriology | BMLS

👩‍⚕️👨‍⚕️ BMLS Students – Important Systematic Bacteriology Test!
In this video, we explain the Decarboxylase Test, a key biochemical test used for bacterial identification based on amino acid decarboxylation. This test is very important for practical exams, viva & routine laboratory diagnosis.

🔬 What is Decarboxylase Test?

🔹 A biochemical test used to detect the ability of bacteria to decarboxylate amino acids
🔹 Produces alkaline amines causing pH change
🔹 Helps differentiate Enterobacteriaceae

📚 Topics Covered in This Video:

📌 Definition of Decarboxylase Test
📌 Principle of decarboxylation reaction ⚗️
📌 Types of Decarboxylase Tests
✔ Lysine Decarboxylase
✔ Ornithine Decarboxylase
✔ Arginine Dihydrolase
📌 Media used 🧫
📌 Step-by-step procedure
📌 Result interpretation (Positive & Negative)
📌 Clinical & diagnostic importance

🧫 Principle:

🧠 Bacteria first ferment glucose → acidic pH
➡️ Anaerobic condition activates decarboxylase enzyme
➡️ Amino acid → Amine + CO₂
➡️ pH becomes alkaline (purple color) 💜

🎨 Indicator Used:

🔹 Bromocresol Purple

✅ Results Interpretation:

🟣 Positive → Purple color (alkaline)
🟡 Negative → Yellow color (acidic)

🦠 Common Organisms:

✔ E. coli
✔ Salmonella
✔ Shigella
✔ Klebsiella
✔ Enterobacter

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🎯 Why This Test is Important?

✔ Identification of Gram-negative bacilli
✔ Differentiation within Enterobacteriaceae
✔ Frequently asked in BMLS exams & viva

👥 Who Should Watch?

👩‍🔬 BMLS Students
👨‍🔬 BMLT / DMLT Students
🎓 Allied Health Science Students
🧫 Microbiology Learners

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Ornithine decarboxylase test
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