Patch Clamp & Perfusion Techniques: Ion Channels Expressed-Xenopus oocytes l Protocol Preview

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Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes - a 2 minute Preview of the Experimental Protocol

Junqiu Yang, Kelli Delaloye, Urvi S. Lee, Jianmin Cui
Washington University in St. Louis, Department of Energy, Environmental & Chemical Engineering; Washington University in St. Louis, Department of Biomedical Engineering; Washington University in St. Louis, Department of Biomedical Engineering and Cardiac Bioelectricity and Arrhythmia Center;

Ionic current of BK channels is recorded using patch clamp techniques. BK channels are expressed in Xenopus oocytes by injecting messenger RNA. The intracellular solution during patch clamp recordings is controlled by a perfusion system.

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