VACV for Quantification of Viral Function during Gene Expression | Protocol Preview

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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression - a 2 minute Preview of the Experimental Protocol

Daniel K. Rozelle, Claire Marie Filone, Ken Dower, John H. Connor
Boston University School of Medicine, Department of Microbiology;

We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.

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