Improving 2D & 3D Skin: Models Using Macromolecular Crowding l Protocol Preview

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Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding - a 2 minute Preview of the Experimental Protocol

Paula Benny, Cedric Badowski, E. Birgitte Lane, Michael Raghunath
National University of Singapore, Department of Biochemistry, Yong Loo Lin School of Medicine; Institute of Medical Biology, A*STAR, Epithelial Biology Laboratory; National University of Singapore, Department of Biomedical Engineering, Faculty of Engineering;

We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.

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