ELISA Blocking Explained: Why and How to Do It Correctly
Автор: EnkiLife Biotech
Загружено: 2026-01-11
Просмотров: 291
Описание:
After coating your ELISA plate, the next critical step is blocking. In this video, we dive into the science behind blocking and walk you through the exact protocol to ensure your assay runs with minimal background and high specificity.
You’ll learn:
Why unoccupied binding sites must be blocked
How blocking reagents prevent non-specific protein adsorption
A clear, step-by-step visual guide to the blocking process
Best practices for handling, incubating, and storing your blocked plates
#ELISA #Blocking #Immunoassay #LifeScience #LabProtocol #Antibody #ProteinBinding #Specificity #BioTech #ResearchMethods
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