Postnatal Cerebellar GC Migration Imaging by Confocal Macroscopy | Protocol Preview

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Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy - a 2 minute Preview of the Experimental Protocol

Magalie Bénard, Alexis Lebon, Hitoshi Komuro, David Vaudry, Ludovic Galas
Inserm, IRIB, University of Rouen, PRIMACEN, Cell Imaging Platform of Normandy; Yale University, Department of Neurobiology, School of Medicine;

During postnatal cerebellum development, immature granule cells originating from the germinal zone exhibit distinct modalities of migration to reach their final destination and to establish neuronal networks. This protocol describes the preparation of cerebellar slices and the confocal macroscopic approach used to investigate the factors that regulate neuronal migration.

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