Cancer Treatment: Isolation & Expansion Of Cytotoxic Killer T Cells

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Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment - a 2 minute Preview of the Experimental Protocol

Chi-Hao Hsiao, Ya-Hsu Chiu, Shao-Chih Chiu, Der-Yang Cho, Liang-Ming Lee, Yu-Ching Wen, Jia-Jhe Li, Ping-Hsiao Shih
Wan Fang Hospital, Department of Urology; Taipei Medical University, Department of Urology, School of Medicine, College of Medicine; Taipei Medical University, Graduate Institute of Clinical Medicine, College of Medicine; China Medical University Hospital, Translational Cell Therapy Center, Department of Medical Research; China Medical University, Graduate Institute of Biomedical Sciences; China Medical University, Graduate Institute of Immunology; China Medical University Hospital, Department of Neurosurgery, Neuropsychiatric Center;

Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3+CD56+ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.

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