Proteoform analysis on the Nautilus Proteome Analysis Platform

Описание: Read our recent preprint to explore novel proteoform data and it's potential to advance basic and applied biology:
https://doi.org/10.1101/2025.06.26.66...

Proteoform analysis on the Nautilus Proteome Analysis Platform

While it's commonly stated that there are about 20,000 proteins encoded in the human genome, these 20,000 proteins can be modified in many ways to produce potentially millions of proteoforms with modified functions, localizations, half-lives, and more. Each proteoform is defined by the full set of modifications it has, whether those modifications come from alternative splicing, post-translational modification, or any other source. Thus identifying proteoforms requires single-molecule analysis of full-length proteins.

Knowing the proteoform makeup of a cell, tissue, or organism may reveal the molecular underpinnings of its biology and enable scientists to develop more effective biomarkers and drugs. In this animation, we show how the Nautilus Proteome Analysis Platform is designed to use Iterative Mapping for single-molecule analysis of intact proteoforms. This may enable in-depth studies of proteoform biology that are not possible on other platforms.

Chapters
00:28 - What are proteoforms?
01:29 - Quantifying proteoforms on the Nautilus Proteome Analysis Platform
02:48 - Applications of proteoform analyses

What aspects of proteoform biology are you particularly excited to learn about? Let us know in the comments below.

Read more about proteoforms on our blog:
https://www.nautilus.bio/blog/what-ar...
https://www.nautilus.bio/blog/targete...

Check out this animation to learn about broadscale proteome analysis on the Nautilus Platform:
   • Quantifying proteins on the Nautilus Prote...