NEET Logical and important question Series
Автор: Logical Botany with Dr. Dharmendra Sharma
Загружено: 2026-01-13
Просмотров: 272
Описание:
Answer: 1. White color colonies will be selected. The plasmid shown contains the gene for \(\beta \)-galactosidase, which acts as a reporter gene for insertional inactivation. When the substrate X-gal is present, an intact \(\beta \)-galactosidase gene produces a blue color in bacterial colonies. The restriction site EcoRI is located within the \(\beta \)-galactosidase gene. When an alien piece of DNA is inserted at the EcoRI site, it disrupts the gene's function (insertional inactivation). As a result, the colonies containing the recombinant plasmid (with the inserted DNA) cannot produce functional \(\beta \)-galactosidase and will appear white in the presence of X-gal. The colonies without the insert will have an intact \(\beta \)-galactosidase gene and will appear blue. Therefore, the white colonies are the desired recombinants.
Molecular Biology
Biotechnology
Genetic Engineering
Cloning
Cloning Vector
Plasmid
#Recombinant DNA
#DNA Cloning
Blue White Screening
Insertional Inactivation
Selectable Marker
LacZ gene
Beta Galactosidase
Transformation
E. coli
Biology Tutorial
Science Education
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