Plasmids | Cloning | Biotechnology | NCERT / NEET Biology | Class 12
Автор: Modelexam
Загружено: 2018-11-21
Просмотров: 443
Описание:
Plasmids
Circular extra chromosomal DNA
Also called vectors
Through which the DNA is injected to the host organism
For example,
If the DNA from Escherichia coli is to be injected
Then the DNA is injected into the plasmid .
And then the plasmid is injected into host organism
DNA replicates and multiplies in host organism
Makes multiple copies of DNA injected
Restriction enzymes and ligases
Restriction enzymes
Cutting DNA at specific region = by molecular scissors
Otherwise called as restriction enzymes
DNA ligases
DNA joined with plasmids = DNA ligases
Genetically modifying an organism
Steps :
DNA with Desirable gene is identified
It is injected into the host
DNA is maintained and multiplied inside the host
DNA is transferred to the progeny
Tools of recombinant dna technology
Host organisms
Restriction enzymes
Polymerase enzymes
Ligases
Vectors
Restriction enzymes
Adds methyl group to DNA
And cuts DNA
Also called restriction endonuclease
First restriction endonuclease is Hind II
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