Library selected AAV variants can effectively translate to non human primates in the spinal cord and
Автор: GenScript USA Inc.
Загружено: 2021-07-28
Просмотров: 268
Описание: Dr. Hanlon discussed his work on the development and characterization of AAV variants with enhanced CNS transduction properties. To discover improved AAV variants, Dr. Hanlon and colleagues created an AAV capsid library by insertion of short random oligonucleotides. Library screening was enabled by iTransduced, a newly developed platform for capsid selection. In iTransduced, a CRE recombinase sequence is inserted together with the random oligonucleotides into the AAV vector, enabling AAV capsid library screening in the context of tdTomato expression in Floxed-STOP tdTomato mice. Identification and selection of brain cells expressing the tdTomato reporter allowed Dr. Hanlon and colleagues to recover by PCR the AAV9 capsid sequence with random oligonucleotide insertions for re-incorporation into the library and a new round of screening.
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